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小鼠颅面组织和未切除骨骼的组织制备及免疫染色

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Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Please note that all translations are automatically generated. Click here for the English version.

小鼠颅面组织和未切除骨骼的组织制备及免疫染色

DOI:

10.3791/59113-v

•

10:03 min

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May 10, 2019

•

Jingwen Yang*^1,2, Haichun Pan*², Yuji Mishina

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology,Wuhan University, ²Department of Biologic and Materials Sciences, School of Dentistry,University of Michigan

Chapters

00:04Title
01:13Preparation of Embryonic Tissues
03:38Preparation of Postnatal Undecalcified Hard Tissues
04:15Cryosectioning
05:18Immunofluorescence Staining
06:50Results: Immunostained Mouse Craniofacial Tissues and Undecalcified Bone
09:03Conclusion

Summary

자동 번역

在这里,我们提出了一个详细的协议,以小鼠颅面组织为例,通过免疫染色来检测和量化颅面形态形成/发病机制期间的蛋白质水平。此外,我们描述了一种制备和冷冻方法,从幼鼠的未分化硬组织免疫染色。

Tags

Immunofluorescence Immunostaining Undecalcified Bone Antigen Retrieval Decalcification Cryoprotection Embedding Freezing Sectioning PBS PFA EDTA Sucrose OCT Compound

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