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动态核极化固态核磁共振法制备结构澄清用真菌和植物材料
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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动态核极化固态核磁共振法制备结构澄清用真菌和植物材料

DOI:
10.3791/59152-v

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09:37 min

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February 12, 2019

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, , , , ,
1Department of Chemistry,Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Chapters

  • 00:04Title
  • 01:2313C, 15N-Labeled Aspergillus fumigatus Liquid and Growth Media Preparation
  • 03:03Fungal Materials Growth
  • 04:21A. fumigatus Preparation for Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP) Studies
  • 06:47Plant Biomass Preparation for DNP Analysis
  • 07:49Results: Fungal Cell Wall Structure Characterization by Solid-State NMR
  • 09:02Conclusion

Summary

자동 번역

자동 번역

提出了一种用于多维固态核磁共振光谱和动态核极化 (dnp) 研究的 13 c、15个n 标记真菌和植物样品的制备方案。

Tags

Fungal MaterialsPlant MaterialsSolid-state NMRDynamic Nuclear PolarizationBiomaterialsCarbohydratesFungal Cell WallsCell CultureYPD MediumLabeled Minimal MediumIsotope LabelingCarbon-13Nitrogen-15SterilizationAutoclaveCell Culture Techniques

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