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1Department of Plant Ecology,Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center,Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing,Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture,Kyushu University, 5Department of Bacteriology,University of Wisconsin, 6Research Faculty of Agriculture,Hokkaido University
Chapters
- 00:04Title
- 01:14Sampling: Tension Relaxation within Water Column of Xylem Conduits
- 03:00Freeze Fixation with Liquid Nitrogen
- 04:04Specimen Preparation
- 05:49Transfer to the Cryo-SEM Specimen Chamber
- 06:30Freeze-etching
- 07:00Results: Xylem Water Distribution in Woody Plants
- 09:04Conclusion
Observar la distribución del agua dentro del xilema proporciona información significativa sobre la dinámica del flujo de agua en las plantas leñosas. En este estudio, demostramos el enfoque práctico para observar la distribución de agua de xilema in situ mediante el uso de un criostato y crio-SEM, que elimina los cambios más recientes en el estado del agua durante la preparación de la muestra.
Tags
Keywords:
XylemWater DistributionCryo-scanning Electron MicroscopeFreeze-fixationXylem TensionWater PotentialCavitationXylem CellsAerotomeWoody PlantsWater RegimeSeasonal VariationBiotic StressFreeze/thaw CycleWet WoodSampling ProcedurePressure ChamberRelaxation TreatmentWater SurfaceTranspirationVessel Length
