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1Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital, 4Center for Perinatal Research,Nationwide Children’s Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery,Nationwide Children’s Hospital
Chapters
- 00:04Title
- 01:15Scaffold Manufacturing
- 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
- 04:40Cell Seeding
- 05:21Graft Implantation
- 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
- 09:27Conclusion
Sténose de la greffe constitue un obstacle critique en remplacement de tissu conçu des voies respiratoires. Afin d’étudier les mécanismes cellulaires qui sous‑tendent la sténose, nous utilisons un modèle murin de remplacement trachéale tissu machiné avec ensemencé la moelle osseuse des cellules mononucléaires (BM-MNC). Ici, nous détaillons notre protocole, y compris la fabrication d’échafaudages, BM-MNC isolement, semis de la greffe et l’implantation.
