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Journal / Bioengineering / 小鼠模型中生物合成组织工程气管移植的育苗与植入…
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Bioengineering

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JoVE Journal Bioengineering
小鼠模型中生物合成组织工程气管移植的育苗与植入
 
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小鼠模型中生物合成组织工程气管移植的育苗与植入

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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接枝狭窄是组织工程气道置换术的关键障碍。为了研究狭窄的细胞机制, 我们利用组织工程气管替代种子骨髓单个核细胞 (BM-MNC) 的小鼠模型。在这里, 我们详细介绍了我们的协议, 包括支架制造、BM-MNC 隔离、接枝播种和植入。

Tags

生物工程 第146期 电纺、合成支架、医药、气管移植、细胞播种、生物材料、气管疾病
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