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1Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital, 4Center for Perinatal Research,Nationwide Children’s Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery,Nationwide Children’s Hospital
Chapters
- 00:04Title
- 01:15Scaffold Manufacturing
- 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
- 04:40Cell Seeding
- 05:21Graft Implantation
- 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
- 09:27Conclusion
Transplantat stenose udgør en afgørende hindring i tissue Engineering luftvejene udskiftning. For at undersøge cellulære mekanismer bag stenose, benytter vi en murine model af væv manipuleret trakeal udskiftning med seedede knoglemarv mononukleære celler (BM-MNC). Her, detalje vi vores protokol, herunder stillads produktion, BM-MNC isolation, graft såning og implantation.
