Journal
/
면역학 및 감염병학
/
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

DOI:
10.3791/59194-v

•

08:42 min

•

April 10, 2019

•

, , , ,
1Department of Pediatrics,Northwestern University, 2Division of Pediatric Surgery,Ann & Robert H. Lurie Children’s Hospital of Chicago

Chapters

  • 00:04Title
  • 01:25Crypt Isolation and Plating from Whole Tissue
  • 05:21Induction of Experimental NEC and Preparation for Paraffin Embedding
  • 07:22Results: Analysis of Enteroids
  • 08:21Conclusion

Summary

자동 번역

자동 번역

Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

Tags

Keywords: Human Epithelial EnteroidsNecrotizing EnterocolitisIntestinal Stem CellsOrganoidsCell IsolationInflammationIntestinal EpitheliumCo-administrationLipopolysaccharide

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/59194?article_type=v

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
check_url/kr/59194?article_type=v

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
check_url/kr/59194?article_type=v

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
check_url/kr/59194?article_type=v

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
check_url/kr/59194?article_type=v

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
check_url/kr/59194?article_type=v

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
check_url/kr/59194?article_type=v

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
check_url/kr/59194?article_type=v

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
check_url/kr/59194?article_type=v

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
check_url/kr/59194?article_type=v

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Read Article