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A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
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A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

DOI:
10.3791/59220-v

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07:52 min

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April 09, 2019

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, , , , , ,
1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS

Chapters

  • 00:04Title
  • 00:36Human Cerebral Microvascular Endothelial Cell (hCMEC)/D3 Insert Coating and Blood Brain Barrier (BBB) Preparation
  • 02:48BBB-Minibrain Endothelial Permeability Validation
  • 03:38BBB Crossing and Multiplication of Yellow Fever Viruses in the Minibrain and Biomolecule BBB Crossing and Brain Cell Targeting
  • 05:22Results: Representative BBB-Minibrain Testing of Neuroinvasive Variants and Biomolecule Properties
  • 07:19Conclusion

Summary

자동 번역

자동 번역

Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells.

Tags

Blood-brain InterfaceBlood-brain BarrierBBBPathogen의학NeurosystemMini-brainEndothelial CellsLucifer YellowPermeabilityTransport BufferCHME Clone Five CellsNeuronsAstrocytesPolyester MembraneCell CultureCell SeedingCell CountingCell Trypsinization

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