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Chapters
- 00:04Title
- 00:34Mesofluidic Device Fabrication
- 02:20Collagen Injection, 3D Imaging, and Quantification
- 04:20Results: Representative Ligand Gradient Evaluation and Cellular Gradient Reponses
- 05:23Conclusion
We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.
