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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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유전학
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

DOI:
10.3791/59235-v

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08:54 min

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March 29, 2019

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, , , , ,
1Center for Epigenetics,Van Andel Research Institute, 2Amgen

Chapters

  • 00:04Title
  • 00:40Overview of the REMOTE-control System
  • 01:50Modify the Gene of Interest for Repression by REMOTE-control
  • 06:04Manipulate Gene Expression In Vivo
  • 06:50Results: In vivo Repression and Activation Capability of the REMOTE-control System
  • 08:23Conclusion

Summary

자동 번역

자동 번역

This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

Tags

Keywords: REMOTE-control SystemEndogenous Gene ExpressionGene FunctionGene RepressionGene UpregulationLacIGY RepressorRtTA-M2 ActivatorIPTGDoxycyclineGene ModificationUCSC Genome Browser

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