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Chapters
- 00:04Title
- 02:01Plasmid Design and Construction
- 03:20HEK-293T Cells Culturing, Plating, and Transfection
- 05:15Virus Harvesting and Viral Particles Concentrating
- 08:16Transduction of MD NPCs
- 09:48Analysis of Methylation Changes
- 11:04Results: Assessment of the LV-dCas9-DNMT3A-GFP/Puro Vectors Compared to the Naive GFP Counterpart
- 12:45Conclusion
