JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:42The Procedure
- 01:41Preparation of the Green Pool
- 02:09Engineering the Green Sub-pools
- 03:19Preparing Borosilicate Needles
- 04:17Disinfecting the Hood and Preparing Cell Tracer
- 04:48Assembling the Injection Tube
- 05:20Mixing “Test” and “Control” Green Sub-Pools
- 06:24Intraventricular Transplantation
- 09:11Histology and Immunoflourescence
- 10:09Morphometry
- 12:52Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
- 14:17Conclusion
Tags
Keywords:
Intraventricular TransplantationEngineered Neuronal PrecursorsIn Vivo Neuroarchitecture StudiesGene Expression ManipulationCortical Neuronal ArchitectureTau EGFP Transgenic EmbryosLentiviral InfectionNeurosphereImmunofluorescenceMorphometric ParametersPrecursor PoolTet-Off SystemDoxycycline
