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Chapters
- 00:00Title
- 00:42The Procedure
- 01:41Preparation of the Green Pool
- 02:09Engineering the Green Sub-pools
- 03:19Preparing Borosilicate Needles
- 04:17Disinfecting the Hood and Preparing Cell Tracer
- 04:48Assembling the Injection Tube
- 05:20Mixing “Test” and “Control” Green Sub-Pools
- 06:24Intraventricular Transplantation
- 09:11Histology and Immunoflourescence
- 10:09Morphometry
- 12:52Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
- 14:17Conclusion
На основе in vitro лентивирной инженерии нейрональных прекурсоров, их совместной трансплантации в мозг дикого типа и парной морфометрической оценки "тест" и "контроль" производных, этот метод позволяет точное моделирование in vivo генного контроля неокортикальных морфология нейронов в простой и доступный способ.
Tags
Keywords:
Intraventricular TransplantationEngineered Neuronal PrecursorsIn Vivo Neuroarchitecture StudiesGene Expression ManipulationCortical Neuronal ArchitectureTau EGFP Transgenic EmbryosLentiviral InfectionNeurosphereImmunofluorescenceMorphometric ParametersPrecursor PoolTet-Off SystemDoxycycline
