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Chapters
- 00:04Title
- 00:32Growth and Synchronization of Nematode Population
- 02:40Hydration of Probes, Drug Loading and Cartridge Equilibration
- 04:40Preparation of Worm Plate, Assay Run, and Post-experiment Data Analysis
- 06:45Results: OCR in Wild Type and Various sel-12 Mutants
- 07:36Conclusion
Mitochondrial respiration is critical for organismal survival; therefore, oxygen consumption rate is an excellent indicator of mitochondrial health. In this protocol, we describe the use of a commercially available respirometer to measure basal and maximal oxygen consumption rates in live, intact, and freely-motile Caenorhabditis elegans.
