In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Changsi Cai; Stefan  A. Zambach; Jonas  C. Fordsmann; Micael L&#248;nstrup; Kirsten J. Thomsen; Aske  G. K. Jensen; Martin Lauritzen

doi:10.3791/59286

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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

DOI:

10.3791/59286-v

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10:54 min

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June 07, 2019

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Changsi Cai, Stefan A. Zambach, Jonas C. Fordsmann, Micael Lønstrup, Kirsten J. Thomsen, Aske G. K. Jensen, Martin Lauritzen²

¹Department of Neuroscience, Faculty of Medicine and Health Science,University of Copenhagen, ²Department of Clinical Neurophysiology,Rigshospitalet

Chapters

00:04Title
00:57Surgical Procedure: Tracheotomy
01:55Surgical Procedure: Catheter Insertion
02:59Surgical Procedure: Craniotomy
05:29Two-photon Imaging
06:32Insertion of the Glass Micro-pipette
09:11Results: ATP Puffing by Micro-pipette Induces Vessel Dilation, followed by Constriction
10:30Conclusion

Summary

자동 번역

We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

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Chapters

Summary

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