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等温滴定量计测定酶稳定性

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Measuring Enzymatic Stability by Isothermal Titration Calorimetry

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等温滴定量计测定酶稳定性

DOI:

10.3791/59302-v

•

08:37 min

•

March 26, 2019

•

W. K. Dindi Chan, Marco Mason, Lee D. Hansen, Jason Donald Kenealey

¹Department of Nutrition, Dietetics and Food Science,Brigham Young University, ²Department of Chemistry and Biochemistry,Brigham Young University

Chapters

00:04Title
00:51Preparing Samples
03:00Preparing the ITC (Isothermal Titration Calorimetry) Instrument
05:22Setting up the ITCrun and Analyzing Data
06:36Results: Enzymatic Stability
08:04Conclusion

Summary

자동 번역

酶活性的热稳定性很容易用等温滴定量热法 (ITC) 测量。目前使用的大多数蛋白质稳定性检测测量蛋白质展开, 但没有提供有关酶活性的信息。通过国贸中心, 可以直接测定酶修饰对酶活性稳定性的影响。

Tags

Keywords: Enzyme Stability Isothermal Titration Calorimetry Enzymatic Reactions Energy-efficient Catalysts Enzyme Modifications Enzyme Assays Sodium Acetate Buffer Enzyme Solution Substrate Solution

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