Journal
/
생화학
/
使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
Please note that all translations are automatically generated. Click here for the English version.

使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体

DOI:
10.3791/59308-v

•

08:37 min

•

June 25, 2019

•

, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

Chapters

  • 00:04Title
  • 01:13Tissue Cutting
  • 01:45Blocking of Nonspecific Binding Sites and Tissue Permeabilization
  • 02:44Incubation with Antibodies
  • 03:18Tissue Mounting
  • 03:56Controls
  • 04:26Image Acquisition
  • 05:31Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
  • 07:18Conclusion

Summary

자동 번역

자동 번역

这里介绍的是免疫组织化学表征和定位的orexin肽,orexin受体,和内分泌大麻素受体在肠道和大脑中正常和饮食诱发肥胖(DIO)成年斑马鱼模型使用免疫渗透酶和双免疫荧光法。

Tags

ImmunohistochemistryImmunoperoxidaseImmunofluorescenceZebrafishOrexinEndocannabinoid ReceptorsCryosectioningBlockingPrimary AntibodiesIncubation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

脂肪酸鉴定<em>蜡状芽孢杆菌</em

植物冰结合蛋白通过冰再结晶抑制和隔离的评估使用冰亲和力纯化鉴定

RNA 结合区质量谱鉴定的样品制备

利用生物测定引导分馏法鉴定海洋鳗信息素

利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白

利用多功能生化方法鉴定新型 ck2 激酶基材

血管紧张蛋白II图解的硝酸分析与鉴定

SUMO 结合实体 （SUBEs） 作为肝癌中 SUMO 蛋白酶的浓缩、分离、识别和表征的工具

分析泛性基质和泛性基质样的依从性转化后修饰和重大改变的识别

使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化

Read Article