/

/

使用 En 面免疫荧光染色直接观察血管内皮细胞

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Please note that all translations are automatically generated. Click here for the English version.

使用 En 面免疫荧光染色直接观察血管内皮细胞

DOI:

10.3791/59325-v

•

00:06 min

•

August 20, 2019

•

Chang Li*^1,2, Zhu Hui Liu*^1,2, Jia Wei Chen², Xin Yi Shu², Ying Shen, Feng Hua Ding, Rui Yan Zhang, Wei Feng Shen, Lin Lu, Xiao Qun Wang

¹Department of Cardiology, Ruijin Hospital,Shanghai Jiao Tong University School of Medicine, ²Institute of Cardiovascular Diseases,Shanghai Jiao Tong University

Chapters

00:04Title
00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
02:47Pretreatment, Immunostaining and Mounting of the Aorta
04:37Results: Representative En Face Staining Images
05:54Conclusion

Summary

자동 번역

在这里，我们提出了免疫荧光染色方案，以直接观察小鼠主尾的内皮细胞。该技术在研究不同流动模式的内皮细胞细胞和分子表型以及动脉粥样硬化的发展时非常有用。

Tags

En Face Immunofluorescence Vascular Endothelial Cells Fluid Shear Stress Aorta Dissection Perfusion Paraformaldehyde Permeabilization Immunostaining VCAM1

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

瓣膜内皮细胞的分离

直接进入成年斑马鱼的肾细胞移植

米色/布里特细胞的间质血管细胞的分离和分化

强大的3D直接标记的探针DNA鱼

后期阶段的胚胎和幼虫的超声，促进免疫荧光染色<em>果蝇</em>组织<em>原位</em

鼠阀门内皮细胞的分离

隔离和鼠标主动脉内皮细胞原代培养

对小鼠乳腺冰冻切片间接免疫荧光

一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层

一个简单的生物测定血管内皮生长因子的评价

Read Article