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Chapters
- 00:04Title
- 01:02Construction of the Plasmids Expressing the Rare-codon-rich Marker Genes
- 01:50Optimizing the Selection Conditions
- 03:23Optimizing the Screening Conditions
- 04:23Identification of the Amino Acid Overproducers
- 07:51Results: Identifying Amino Acid Overproducers
- 09:43Conclusion
Tags
Amino Acid OverproducersRare-codon-rich MarkersAnalog-based ApproachHigh ThroughputTranslation Of Rare CodonsMolecular Experimental OperationsSelection StringenciesVisual DemonstrationSelection And Screening SystemKanRRare Codon CTAWild-type Selection Marker GeneRare-codon-rich DerivativeOD600L-leucine
