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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Chapters
- 00:04Title
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
Dit protocol presenteert een gestandaardiseerde methode om te groeien VX2 cellen in de cultuur en om een orthotopic VX2 model van endometriumkanker met retroperitoneale lymfeklier metastasen bij konijnen te creëren. Orthotopic endometriumkanker modellen zijn belangrijk voor de pre-klinische studie van nieuwe beeldvormings modaliteiten voor de diagnose van lymfeklier metastasen.
