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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Chapters
- 00:04Title
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
इस प्रोटोकॉल संस्कृति में VX2 कोशिकाओं को विकसित करने के लिए और खरगोशों में retroperitoneal लिम्फ नोड metastases के साथ एंडोमेट्रियल कैंसर के एक orthotopic VX2 मॉडल बनाने के लिए एक मानकीकृत विधि प्रस्तुत करता है। ऑर्थोटोपिक एंडोमेट्रियल कैंसर मॉडल लिम्फ नोड मेटास्टेसिस के निदान के लिए उपन्यास इमेजिंग तौर तरीकों के पूर्व नैदानिक अध्ययन के लिए महत्वपूर्ण हैं।
