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一种新的生理免疫原树突状细胞生成方法
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
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一种新的生理免疫原树突状细胞生成方法

DOI:
10.3791/59370-v

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12:08 min

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May 17, 2019

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, , , , , , , , , , ,
1Department of Dermatology,Yale University School of Medicine, 2Dermatology Department,University of Rome Tor Vergata, 3Department of Pathology,Yale University School of Medicine

Chapters

  • 00:04Title
  • 01:37Collection of Peripheral Blood Mononuclear Cells for Transimmunization
  • 03:098-MOP/UVA Treatment of Tumor Cells
  • 04:26TI Plate Passage of Cells
  • 08:12Overnight Co-incubation of PBMC with Antigen
  • 09:19Results: Mouse-to-man Scalable Miniature ECP Device for Cancer Immunotherapy
  • 11:07Conclusion

Summary

자동 번역

자동 번역

开发了转移 (TI) 装置或平板和相关的协议, 以便在实验环境中复制体外光化疗 (ECP) 的主要特征, 从而产生生理激活、可调谐的树突状用于癌症免疫治疗的细胞 (Dc)。

Tags

Dendritic CellsPhysiologic Dendritic Antigen-presenting CellsPhDCMonocyte-to-DC ConversionPlatelet SignalingAnti-tumor T Cell ResponsesMonocyte-to-PhDC MaturationPeripheral Blood Mononuclear CellsPBMCRed Blood Cell LysisTumor-bearing Mice

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