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Chapters
- 00:04Introduction
- 00:52Brain Punching
- 02:20Co-extraction of eCBs and eiCs
- 04:32Co-extraction of PLs, eCBs and Dual Extraction of RNA and Lipids
- 07:51Results: Lipidomic and Transcription Profiling in KA-induced Epilepsy
- 09:27Conclusion
This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined.
