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CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术
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CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
DOI:

07:44 min

September 14, 2019

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Chapters

  • 00:04Title
  • 01:45Selecting and Harvesting ES-like Cells
  • 03:02Deletion of Exon 23 in Mouse iPSCs with Two Guide RNAs (gRNAs) coupled with Cas9
  • 05:25Identification of iPSC Colonies with Exon23 Deletion
  • 06:33Results: CRISPR/Cas9-mediated Exon23 Deletion
  • 06:57Conclusion

Summary

자동 번역

在这里,我们提出了基于Cas9的外显23删除协议,从Dmdmdx小鼠衍生的皮肤成纤维细胞恢复iPSC中的营养不良素表达,并使用Tet-on MyoD激活系统将iPSC直接分化为肌源性祖细胞(MPC)。

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