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Chapters
- 00:04Title
- 01:45Selecting and Harvesting ES-like Cells
- 03:02Deletion of Exon 23 in Mouse iPSCs with Two Guide RNAs (gRNAs) coupled with Cas9
- 05:25Identification of iPSC Colonies with Exon23 Deletion
- 06:33Results: CRISPR/Cas9-mediated Exon23 Deletion
- 06:57Conclusion
Aquí, presentamos un protocolo de eliminación exon23 basado en Cas9 para restaurar la expresión de distrofina en iPSC a partir de fibroblastos cutáneos derivados del ratón Dmdmdx y diferenciar directamente los iPSC en células progenitoras miogénicas (MPC) utilizando el sistema de activación Tet-on MyoD.
