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Chapters
- 00:04Title
- 00:36Mouse Preparation
- 01:12Pharmacological Inactive X Chromosome (Xi) Reactivation
- 02:50Brain Harvest
- 04:45Cryo-sectioning
- 05:35Xi Reactivation Determination
- 06:34Results: Representative Xi-Mecp2 Validation and Pharmacological Reactivation
- 07:52Conclusion
Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.
