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解冻、培养和冷冻保存的嗜酸性粒细胞系

JoVE 신문
발생학

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JoVE 신문 발생학

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

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解冻、培养和冷冻保存的嗜酸性粒细胞系

DOI:

10.3791/59459-v

•

07:57 min

•

April 16, 2019

•

Arthur Luhur, Kristin M. Klueg, Johnny Roberts, Andrew C. Zelhof

¹Drosophila Genomics Resource Center, Department of Biology,Indiana University Bloomington

Chapters

00:04Title
00:21Frozen Drosophila Cell Line Thawing and Reviving
02:01Semi-adherent Cell Subculture
03:26Adherent Cells Detachment
04:33Manual Cell Counting
05:23Drosophila Cell Line Cryopreservation
06:41Results: Representative Images of Distinct Drosophila Cell Lines
07:30Conclusion

Summary

자동 번역

食子细胞系是基础和生物医学研究的重要试剂。本文提供了解冻, 亚培养, 冷冻保存常用的嗜食子细胞系, 以协助研究人员将这些试剂的使用纳入他们的研究。

Tags

Keywords: Drosophila Cell Lines Thawing Culturing Cryopreserving Cell Culture Cell Suspension Cell Density Cell Proliferation Cell Viability Cell Morphology Laminar Flow Hood Cell Culture Media Centrifugation Cell Passage Microorganismal Contamination

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