/

의학

/

下颌骨和腮腺表型模型对组织型培养的放射治疗是机体活力特征的关键

JoVE 신문
의학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 의학

Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

Please note that all translations are automatically generated. Click here for the English version.

下颌骨和腮腺表型模型对组织型培养的放射治疗是机体活力特征的关键

DOI:

10.3791/59484-v

•

07:38 min

•

May 17, 2019

•

Rachel Meyer, Wen Yu Wong, Roberto Guzman, Randy Burd, Kirsten Limesand²

¹Department of Nutritional Sciences,University of Arizona, ²Cancer Biology Graduate Interdisciplinary Program,University of Arizona, ³Department of Chemical Engineering,University of Arizona

Chapters

00:04Title
01:02Vibratome Preparation
01:50Tissue Sample Preparation and Sectioning
03:19Salivary Gland Section Culture
04:01Salivary Gland Section Irradiation and Antibody Staining
05:17Results: Representative Characteristics of In Vivo Salivary Gland Section 2D Cultures
06:53Conclusion

Summary

자동 번역

利用三维有机型培养物来可视化唾液腺的形态和功能标记, 可以为辐射后组织损伤的机制提供新的见解。这里描述的是一个协议的部分, 培养, 辐照, 染色, 和图像50-90 微米厚的唾液腺部分之前和之后的电离辐射照射。

Tags

Keywords: Organotypic Culture Submandibular Salivary Gland Parotid Salivary Gland Radiation Treatment Ex Vivo Vibratome Agarose Embedding Cell-cell Interactions Therapeutic Screening Salivary Gland Damage Radiotherapy Gene Manipulation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

沃顿商学院的导管插管通过小鼠颌下腺唾液腺

立体定向颅内植入和<em在体内</em生物发光成像的肿瘤异种移植的小鼠模型系统的多形性胶质母细胞瘤

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

研究胰腺癌干细胞特性开发新的治疗策略

Retroductal颌下腺灌输和本地化分割照射在涎腺功能减退症大鼠模型

Microtumors使用3D人体Biogel培养体系和病人源性胶质母细胞瘤的Kinomic剖析和药物反应测试的生成

从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析

放射规划助理-一个流线型的全自动放疗治疗计划系统

小鼠涎腺照射后通过匹属刺激进行唾液功能评估

角膜上皮机械和化学损伤的离体和体内动物模型

Read Article