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CRISPR/Cas9 核糖核酸蛋白介导的精确基因编辑通过管电穿孔

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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

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CRISPR/Cas9 核糖核酸蛋白介导的精确基因编辑通过管电穿孔

DOI:

10.3791/59512-v

•

08:31 min

•

June 20, 2019

•

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen, Jun Song, Dongshan Yang, Jifeng Zhang, Y. Eugene Chen, Jie Xu

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine,University of Michigan Medical Center, ²Celetrix LLC

Chapters

00:04Title
01:08Preparation of Cells
02:51Tube Electroporation of Cas9 RNP and ssODNs
04:24Analysis of Gene Editing Events
05:48Results: Results of Tube Electroporation
07:59Conclusion

Summary

자동 번역

这里介绍的是使用管电穿孔在哺乳动物细胞中高效CRISPR/Cas9核苷酸介导基因编辑的协议。

Tags

Keywords: CRISPR/Cas9 Gene Editing Tube Electroporation Cell Transfection IPSCs Rabbit Fibroblasts GRNA Donor Oligos Cell Culture Electroporation Buffer

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