Journal
/
생화학
/
从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Please note that all translations are automatically generated. Click here for the English version.

从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂

DOI:
10.3791/59538-v

•

11:59 min

•

May 15, 2019

•

, ,
1Department of Microbiology and Molecular Genetics,Michigan State University, 2Department of Physiology,Michigan State University

Chapters

  • 00:04Title
  • 01:01Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk
  • 02:04Isolation of LDL Particles from Plasma
  • 03:16Incubation of S. aureus with LDLs for Membrane Lipid Analysis
  • 05:18Extraction of S. aureus Membrane Lipids
  • 06:54Analysis of S. aureus Lipid Profiles using High Resolution/Accurate Mass Spectrometry
  • 08:26Database Searching to Identify Endogenous S. aureus and Exogenous LDL-derived Lipids
  • 09:54Results: Fatty Acid Composition of S. aureus Phospholipids Cultured with Chicken LDLs
  • 11:29Conclusion

Summary

자동 번역

자동 번역

该方法为研究将来自复杂宿主来源的外源脂肪酸结合到细菌膜中提供了一个框架,特别是金黄色葡萄球菌。为此,介绍了从鸡蛋蛋黄中浓缩脂蛋白颗粒的方案,以及利用质谱法对细菌磷脂进行随后脂肪酸分析的介绍。

Tags

Lipoprotein ParticlesChicken Egg YolkBacterial PathogenFatty Acid IncorporationMembrane PhospholipidsLipidomic AnalysisStaphylococcus AureusAmmonium SulfateDialysis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

细菌内膜显示筛查抗体片段的图书馆

脂肪酸鉴定<em>蜡状芽孢杆菌</em

使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em

脂肪酸13C Isotopologue 分析提供了对无脊椎动物消费者营养碳转移和脂质代谢的洞察力

HEK 细胞 Mitoribosome 的快速分离

细胞细胞接触物蛋白质-蛋白质相互作用的荧光波动光谱测定

电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定

微 Rna 对原生脂蛋白颗粒的富集及其与绝对相对微 Rna 含量及细胞转移速率的测定

PeptiQuick，将膜蛋白一步进生物仿入肽，用于简化蛋白质结合测定

膜蛋白共翻译插入预成型纳米盘

Read Article