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JoVE Journal Cancer Research
Droplet Digital TRAP (ddTRAP)
 

Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Article DOI: 10.3791/59550-v • 06:38 min • May 3rd, 2019
May 3rd, 2019 •
1, 1, 1
1School of Kinesiology, University of Michigan

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We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.

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Keywords: DdTRAP Telomere Repeat Amplification Protocol Droplet Digital PCR Telomerase Activity Cell Lysis Extension Reaction DdPCR Master Mix Droplet Generation
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