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鼠心围细胞的分离与纯化

JoVE 신문
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Isolation and Purification of Murine Cardiac Pericytes

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鼠心围细胞的分离与纯化

DOI:

10.3791/59571-v

•

10:49 min

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August 16, 2019

•

Linda L. Lee, Aarif Y. Khakoo, Vishnu Chintalgattu

¹Department of Cardiometabolic Disorders,Amgen Research and Discovery, Amgen Inc., ²Department of Translational Sciences,Amgen Research and Discovery, Amgen Inc.

Chapters

00:04Title
00:44Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
03:28Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
06:34Purification of Pericytes from Crude Cell Mixture Using FACS
08:18Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
09:55Conclusion

Summary

자동 번역

我们优化了一个方案，以分离和纯化鼠心脏围细胞，用于基础研究和研究其生物学和治疗潜力。

Tags

Cardiac Pericytes Isolation Purification Mouse Model Cell Sorting Cell Culture

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