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确定小鼠肝脏中的胆汁密度

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Determining Bile Duct Density in the Mouse Liver

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确定小鼠肝脏中的胆汁密度

DOI:

10.3791/59587-v

•

07:35 min

•

April 30, 2019

•

Joshua M. Adams^2,3, Hamed Jafar-Nejad³

¹Program in Developmental Biology,Baylor College of Medicine, ²Medical Scientist Training Program (MSTP),Baylor College of Medicine, ³Department of Molecular and Human Genetics,Baylor College of Medicine

Chapters

00:04Title
00:36Mouse Liver Tissue Collection
01:19Fixation, Paraffin Embedding, and Liver Block Sectioning
02:35Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
04:08Imaging and Quantification of Bile Ducts (BDs)
05:27Results: Determining BD to PV Ratio
07:00Conclusion

Summary

자동 번역

我们提出了一种相当简单和敏感的方法,用于准确定量小鼠肝脏中的胆管密度。该方法有助于确定遗传和环境修饰剂的影响,以及潜在疗法在胆汁疾病小鼠模型中的有效性。

Tags

Bile Duct Density Mouse Liver Liver Disease Genetic Modifiers Bile Duct Development Immunohistochemistry Paraffin Embedding Liver Sectioning Antigen Retrieval

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