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Chapters
- 00:04Title
- 00:59Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
- 02:10LG Single-cell Suspension Preparation
- 04:46Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
- 05:45Results: Representative MEC and Pericyte Characterization and Isolation
- 06:38Conclusion
The lacrimal gland (LG) has two cell types expressing α-smooth muscle actin (αSMA): myoepithelial cells (MECs) and pericytes. MECs are of ectodermal origin, found in many glandular tissues, while pericytes are vascular smooth muscle cells of endodermal origin. This protocol isolates MECs and pericytes from murine LGs.
