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血管化异位脾移植小鼠模型在脾细胞生物学和移植免疫研究中的应用

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A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

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血管化异位脾移植小鼠模型在脾细胞生物学和移植免疫研究中的应用

DOI:

10.3791/59616-v

•

08:04 min

•

June 11, 2019

•

Jiao-Jing Wang*¹, Longhui Qiu*¹, Ramiro Fernandez, Xin Yi Yeap, Charlie Xiaoying Lin, Zheng Jenny Zhang²

¹Comprehensive Transplant Center,Northwestern University, ²Department of surgery, Feinberg School of Medicine,Northwestern University, ³Weinberg art and science college,Northwestern University

Chapters

00:04Title
00:39Donor Spleen Harvest
03:06Recipient Splenectomy and Spleen Graft Implantation
06:28Results: Representative Syngeneic Spleen Transplantation Chimera Generation
07:28Conclusion

Summary

자동 번역

该协议详细介绍了血管化异位脾移植小鼠模型的手术步骤, 这是一种技术上具有挑战性的模型, 可作为研究脾脏细胞命运和寿命的有力工具, 而脾脏是不同脾脏的机制细胞群在疾病进展, 和移植免疫。

Tags

Keywords: Mouse Model Vascularized Heterotopic Spleen Transplantation Spleen Cell Biology Transplant Immunity Congenic Mice Spleen Cells Inflammatory Disease Surgical Procedure Donor Mouse Abdominal Surgery Spleen Graft Heparinized Saline Celiac Artery Portal Vein Splenic Vein

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