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Biochemistry
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JoVE Journal Biochemistry
Oversættelse af Ribosome affinitets rensning (TRAP) for RNA-isolation fra endotelceller in vivo
 
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Oversættelse af Ribosome affinitets rensning (TRAP) for RNA-isolation fra endotelceller in vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Vi præsenterer en tilgang til at rense ribosome-bundet mRNA fra vaskulære endotelceller (ECs) direkte i mus hjerne, lunge og hjerte væv via EF-specifik genetisk tag af forstærket grønt fluorescens protein (EGFP) i ribosomer i kombination med RNA rensning .

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Biokemi angiogenese arteriolær differentiering vaskulære endotelceller oversætte ribosom affinitet rensning RNA ekstraktion realtid qPCR
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