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면역학 및 감염병학

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从小鼠多个器官分离和定量寨卡病毒

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

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从小鼠多个器官分离和定量寨卡病毒

DOI:

10.3791/59632-v

•

10:31 min

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August 15, 2019

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James D. Brien*¹, Mariah Hassert*¹, E. Taylor Stone, Elizabeth Geerling, Lillian Cruz-Orengo, Amelia K. Pinto

¹Department of Molecular Microbiology and Immunology,Saint Louis University, ²School of Veterinary Medicine University of California Davis

Chapters

00:04Title
01:28Organ Isolation
03:06Zika Virus Focus Forming Assay (FFA)
08:05Results: Viral Replication and Focus Forming Assay
09:39Conclusion

Summary

자동 번역

该协议的目的是通过分离和量化寨卡病毒,从感染后小鼠的多个器官中分离和量化,来展示用于调查病毒性疾病的技术。

Tags

Keywords: Zika Virus Viral Infection Mouse Model Central Nervous System Organ Harvesting Viral Pathogenesis Viral Spread Immunological Response Flavivirus Alphavirus Dengue Virus Chikungunya Virus Viral Quantification

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