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1Department of Anatomy and Structural Biology,Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, 3Integrated Imaging Program,Albert Einstein College of Medicine, 4Department of Surgery,Montefiore Medical Center, 5Department of Pathology,Montefiore Medical Center
Chapters
- 00:04Title
- 00:55Transplantation of Syngeneic Breast Tumors into Mouse Hosts with Fluorescent Macrophages
- 02:38Preparation of Mouse for Imaging
- 04:25Evaluation of Extravascular Dextran Using Fixed Tissue Analysis
- 07:10Results: Visualization of Vasculature in Healthy and Diseased Tissues and TMEM-Mediated Vascular Permeability
- 08:56Conclusion
Vi beskriver to metoder til vurdering af forbigående vaskulær permeabilitet forbundet med tumor mikromiljø af metastase (TMEM) døråbning funktion og cancer celle intravasation ved hjælp af intravenøs injektion af høj molekylvægt (155 kDa) dextran i mus. Metoderne omfatter intravital billeddannelse i levende dyr og fast vævsanalyse ved hjælp af immunofluorescens.
