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1Department of Anatomy and Structural Biology,Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, 3Integrated Imaging Program,Albert Einstein College of Medicine, 4Department of Surgery,Montefiore Medical Center, 5Department of Pathology,Montefiore Medical Center
Chapters
- 00:04Title
- 00:55Transplantation of Syngeneic Breast Tumors into Mouse Hosts with Fluorescent Macrophages
- 02:38Preparation of Mouse for Imaging
- 04:25Evaluation of Extravascular Dextran Using Fixed Tissue Analysis
- 07:10Results: Visualization of Vasculature in Healthy and Diseased Tissues and TMEM-Mediated Vascular Permeability
- 08:56Conclusion
Describimos dos métodos para evaluar la permeabilidad vascular transitoria asociada con el microambiente tumoral de la función de la puerta de metástasis (TMEM) y la intravasación de células cancerosas mediante inyección intravenosa de dextran de alto peso molecular (155 kDa) en ratones. Los métodos incluyen imágenes intravitales en animales vivos y análisis de tejido fijo utilizando inmunofluorescencia.
