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Photostimulation av Femtosecond laser aktiverar extracellulär-signalreglerade Kinas (ERK) signalering eller mitokondriella händelser i målceller
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JoVE 신문 생물학
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
DOI:

11:00 min

July 06, 2019

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Chapters

  • 00:04Title
  • 01:04Setting Up the Photostimulation System
  • 01:48Preparing for Activation of ERK2 by Photostimulation
  • 02:47Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode
  • 04:24Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode
  • 05:53Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation
  • 06:55Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode
  • 08:32Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells
  • 10:26Conclusion

Summary

자동 번역

Tätt fokuserad femtosecondlaser laser kan leverera exakt stimulering till celler genom att kopplas till en konfokal mikroskopi möjliggör realtid observation och photostimulation. Den photostimulation kan aktivera cell molekylära händelser inklusive ERK signalering väg och mitokondriella blixtar av reaktiva syreradikaler.

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