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标准化和可扩展的测定,以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
DOI:

10:47 min

November 06, 2019

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Chapters

  • 00:04Title
  • 01:12Preparation of Device, Gel, and Coating
  • 02:42Cell Seeding/Microvessel Culture
  • 04:37Study Angiogenic Sprouting
  • 05:49Study Anastomosis and Sprout Stabilization
  • 06:50Fixation, Staining, and Imaging
  • 08:55Results: Cell Seeding and 3D Sprouting of iPSC-EC
  • 10:13Conclusion

Summary

자동 번역

该方法将iPSC衍生内皮细胞的培养描述为标准化微流体平台中的40个注入3D微容器。该平台支持在3D中研究梯度驱动的血管生成,包括以可扩展的高通量方式血管生成芽的血管生成和稳定。

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