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近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达
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Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain
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近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达

DOI:
10.3791/59685-v

•

06:04 min

•

May 23, 2019

•

, , ,
1Department of Psychological and Brain Sciences,University of Delaware, 2Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Chapters

  • 00:04Title
  • 00:45Single Immunohistochemical Reaction
  • 02:45Imaging and Protein Expression Analysis
  • 04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
  • 05:30Conclusion

Summary

자동 번역

자동 번역

在这里, 我们提出了一个协议, 使用近红外染料结合免疫组织化学和高分辨率扫描来检测大脑区域的蛋白质。

Tags

Near-infrared FluorescenceHigh-resolution ScanningProtein ExpressionRodent BrainImmunohistochemistryMolecular Signaling PathwaysAMPA/NMDA RatioCryostatParaformaldehydeTBSPrimary AntibodySecondary AntibodyNear-infrared Scanning

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