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JoVE Journal Cancer Research
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
 

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Article DOI: 10.3791/59696-v • 10:03 min • July 5th, 2019
July 5th, 2019 •
*1, *1, *1, *2, *1, *2, *2, 1,2,3,4
1Department of Biomedical Engineering, University of Michigan, 2Department of Materials Science and Engineering, University of Michigan, 3Rogel Cancer Center, School of Medicine, University of Michigan, 4Macromolecular Science and Engineering, University of Michigan
* These authors contributed equally

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요약
Automatic Translation

This protocol describes generation of patient-derived spheroids, and downstream analysis including quantification of proliferation, cytotoxicity testing, flow cytometry, immunofluorescence staining and confocal imaging, in order to assess drug candidates’ potential as anti-neoplastic therapeutics. This protocol supports precision medicine with identification of specific drugs for each patient and stage of disease.

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Keywords: 3D Cell Culture Spheroids Hanging Drop Model Anti-neoplastic Drug Screening Cancer Stem Cells Patient-derived Samples Heterogeneity Chemo Resistance Ovarian Cancer Targeted Therapies Patient-specific Treatments High-throughput Screening
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