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Chapters
- 00:04Title
- 00:46Protocol 1: Climbing Assay
- 01:41Protocol 3: Histology Analysis
- 04:32Protocol 4: Gene Mapping of Recessive Mutant Phenotype
- 06:17Protocol 5: DNA Sequencing and Analysis
- 07:08Protocol 6: Further Analysis of Candidate Gene Function
- 07:46Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
- 09:11Conclusion
We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.
