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1Center for Genetic Medicine,Children’s National Health System, 2Institute for Biomedical Sciences,The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology,University of California San Francisco, 5DIPG Centre of Expertise Zurich,Universitats-Kinderspital Zurich
Chapters
- 00:04Title
- 00:59Target DNA Detection and Quantification
- 03:19Data Analysis
- 05:00Results: Representative Mutation Analyses in Pre-amplified Plasma and Cerebrospinal Fluid (CSF) Cell Free DNA (cfDNA)
- 06:32Conclusion
Her presenterer vi en protokoll for å oppdage tumor somatiske mutasjoner i sirkulerende DNA tilstede i pasientens biologiske væsker (biofluids). Vår dråpe Digital polymerase kjedere reaksjon (dPCR)-basert metode muliggjør kvantifisering av tumor mutasjon allel frekvens (MAF), tilrettelegging en minimalt invasiv supplement til diagnostisering og timelig overvåking av tumor respons.
