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An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
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생화학
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JoVE 신문 생화학
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

DOI:
10.3791/59722-v

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07:55 min

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July 06, 2019

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, ,
1Macromolecular Crystallography (HZB-MX),Helmholtz-Zentrum Berlin, 2Department Sample Environment,Helmholtz-Zentrum Berlin

Chapters

  • 00:04Title
  • 00:37Setting up Crystallization Drops and Observing Crystal Growth
  • 01:56Crystal Manipulation
  • 04:23In Situ Diffraction Data Collection at Ambient Temperature and at Cryogenic Temperature
  • 06:20Results: Novel Sample Holder in Macromolecular X-Ray Crystallography for Crystal Growth, Crystal Manipulation, and X-Ray Diffraction Data Collection
  • 07:23Conclusion

Summary

자동 번역

자동 번역

A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.

Tags

Keywords: Macromolecular X-ray CrystallographySample Holder24-well PlateMinimal Background ScatteringCrystal Growth MonitoringCryoprotectionPaper WickCOC FoilPolyimide Foil

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