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巨噬细胞吞噬胸腺细胞吞噬的实验分析

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

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巨噬细胞吞噬胸腺细胞吞噬的实验分析

DOI:

10.3791/59731-v

•

06:47 min

•

May 24, 2019

•

Yuxuan Zhen, Wen-Hai Shao

¹Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine,University of Cincinnati

Chapters

00:04Title
00:36Apoptotic Thymocyte Carboxyfluorescein Succinimidyl Ester (CFSE)-labeling
02:39Peritoneal Macrophage Preparation
03:53Peritoneal Macrophage: Apoptotic Thymocyte Co-culture
04:36Results: Representative Macrophage Phagocytosis and Efferocytosis Analyses
06:12Conclusion

Summary

자동 번역

在这里, 我们提出了一个方案, 以编制凋亡胸腺细胞和腹膜巨噬细胞, 并分析了细胞内吞的效率和特定的抑制介导的抑制凋亡胸腺细胞吞没。该协议在包括人工珠子和细菌在内的其他颗粒的细胞介导清除中有着广泛的应用。

Tags

Keywords: Apoptotic Cells Thymocyte Engulfment Macrophages Phagocytic Cell Types Apoptotic Cell Binding Cell Debris Cell Clearance CFSE Labeling Staurosporine Peritoneal Macrophages Thioglycollate Cell Isolation

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