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Chapters
- 00:04Title
- 00:57Tibialis Anterior (TA) Muscle Collection
- 03:03Cryosectioning
- 03:51Immunolabelling
- 05:03Results: Representative IgG Uptake Staining in a Degenerating Mdx Muscle Cross-Section
- 06:08Conclusion
Described here is a protocol for direct immunolabelling of necrotic myofibers in muscle cryosections. Necrotic cells are permeable to serum proteins, including immunoglobulin G (IgG). Revealing the uptake of IgG by myofibers allows the identification and quantification of myofibers that undergo necrosis regardless of muscle condition.
