This content is Free Access.
Chapters
- 00:04Title
- 00:25Day 1: Picking Hermaphrodites for Mating and Staining of Males
- 01:49Day 2: Mating
- 03:56Day 2: Mounting Worms and Image/Video Acquisition
- 05:17Quantification of Sperm Distribution and Migration
- 07:47Results: Sperm Distribution and Migration
- 09:14Conclusion
Sperm must successfully navigate through the oviduct to fertilize an oocyte. Here, we describe an assay for measuring sperm migration within the C. elegans hermaphrodite uterus. This assay can provide quantitative data on sperm distribution within the uterus after mating, as well as on speed, directional velocity, and reversal frequency.
