Journal
/
암 연구학
/
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

DOI:
10.3791/59795-v

•

07:49 min

•

July 17, 2019

•

, , , , ,
1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research,National Cancer Centre Singapore, 2The Cancer and Stem Cell Program,Duke-NUS Medical School

Chapters

  • 00:04Title
  • 00:21In Vitro Kinase Assay
  • 03:08RAF Co-activation Assay
  • 04:14Complimentary Split Luciferase Assay
  • 05:53Results: RAF Family Kinase Disease-related Mutant Characterization
  • 07:13Conclusion

Summary

자동 번역

자동 번역

In this article, we presented a set of practical and feasible methods for characterizing disease-related mutants of RAF family kinases, which include in vitro kinase assay, RAF co-activation assay, and complementary split luciferase assay.

Tags

Keywords: RAF KinaseDisease-related MutantsCharacterizationCatalytic ActivityFlag-taggedGlutathione S-transferase293T CellsLysis BufferAnti-FLAG Affinity BeadsKinase ReactionSDS-PAGEImmunoblot

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/59795?article_type=v

A Mouse Model of Fatigue Induced by Peripheral Irradiation
check_url/kr/59795?article_type=v

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
check_url/kr/59795?article_type=v

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator
check_url/kr/59795?article_type=v

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
check_url/kr/59795?article_type=v

Detection of Protease Activity by Fluorescent Peptide Zymography
check_url/kr/59795?article_type=v

A Practical Guide for the Production and PET/CT Imaging of 68Ga-DOTATATE for Neuroendocrine Tumors in Daily Clinical Practice
check_url/kr/59795?article_type=v

In Vitro Assay to Study Tumor-macrophage Interaction
check_url/kr/59795?article_type=v

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
check_url/kr/59795?article_type=v

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model
check_url/kr/59795?article_type=v

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Read Article